Cloned (Comment) | Organism |
---|---|
into the vector pGEX6p3 for expression in Escherichia coli cells | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ko095, an embryonic lethal mutant with abnormal branching in the head and trunk blood vessels, the gene responsible for ko095 encodes seryl-tRNA synthetase with a nonsense mutation | Danio rerio |
T429A | the abnormal branching of intersegmental vessels in ko095 mutant is suppressed by the introduction of either wild-type or mutant T429A seryl-tRNA synthetase, T429A lacks the enzymatic activity that catalyzes aminoacylation of transfer RNA for serine | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Danio rerio | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q6DRC0 | - |
- |
Purification (Comment) | Organism |
---|---|
by glutathione Sepharose beads | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Danio rerio | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Seryl-tRNA synthetase | - |
Danio rerio |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
aminoacylation assay at room temperature | Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
aminoacylation assay | Danio rerio |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Danio rerio |
General Information | Comment | Organism |
---|---|---|
physiological function | seryl-tRNA synthetase is involved in vascular development | Danio rerio |